Medical Microbiology: Pseudomonas & Acinetobacter and Other Uncommon Gram Negatives MCQ

Multiple Choice Question on Pseudomonas & Acinetobacter
1) Which one of the given statements is not true about Pseudomonads?
a) Rod-shaped, often curved
b) Motile
c) Found in soil and water and plants
d) All species cause diseases in humans only

2) Pseudomonas aeruginosa produces a water-soluble blue color pigment called pyocyanin and …............ color pigment pyoverdin.
a) Green
b) Blue-green
c) Red
d) Pink-red

3) Patients with cystic fibrosis infection suffer from a chronic lung infection caused by Pseudomonas aeruginosa. The bacterial growth results in …........formation and clogs the lung airways.
a) Pigment
b) Biofilm
c) White blood cells
d) Endotoxin

4) Isolation of Pseudomonas aeruginosa from a mucoid sputum specimen obtained from a patient with cystic fibrosis is usually done by standard culture method. After the incubation, mucoid bacterial colonies can be seen on the agar media which have a grape-like odor, what are the best growth temperature and incubation period for the given bacteria?
a) 42 degree Celsius for 48 hours
b) 37 degree Celsius for 48 hours
c) 42 degree Celsius for 24 hours
d) 37 degree Celsius for 42 hours

5) All of the following statements for Pseudomonas aeruginosa are true, except;
a) It is oxidase positive
b) It does not grow well at 42c
c) It is an opportunistic pathogen
d) It contains fimbriae

7) Pseudomonas aeruginosa is frequently associated with the hospital-acquired bacterial infection, mostly affecting immunocompromised patients. Which of the following is/are example/examples of the various means by which bacteria can transfer and cause infection to the susceptible patient?
a) Healthcare workers
b) Contaminated equipment
c) Air droplets
d) All of the above

8) Multiple virulence factors play an important role in the mechanism of pathogenesis by Pseudomonas aeruginosa, one of the main virulent structural components is pili, which helps the bacteria to adhere to the cell surfaces. Which one is not the virulence factor Pseudomonas aeruginosa?
a) Hemolysins
b) Pigments
c) Phospholipase B
d) Extracellular protease

9) Apart from Pseudomonas aeruginosa, which one of the following gram-negative bacteria can be frequently associated with nosocomial infections?
a) Staphylococcus aureus
b) Candida albicans
c) Eikenella corrodens
d) Acinetobacter baumannii

10) Which one of the following is not the general characteristic of the HACEK ( Haemophilus, Aggregatibacter, Cardiobacterium, Eikinella, Kingella) organisms?
a) Normal flora of the oral cavity
b) Require CO2 for growth
c) Grow well on MacConkey agar
d) Causative agents of infectious endocarditis

11) Burkholderia pseudomallei is aerobic, gram-negative, motile, aerobic, saprophytic bacteria. It grows at 42-degree celsius in standard agar media forming mucoid colonies, cream to orange in color.
a) Pseudomonas aeruginosa
b) Burkholderia mallie
c) Acinetobacter baumannii
d) Burkholderia pseudomallei

12) Which are the most common bacteria found in a specimen taken from burned patients?
a) Streptococcus epidermis
b) Pseudomonas aeruginosa
c) Acinetobacter baumannii
d) Moraxella catarahalis

13) Acinetobacter baumannii is one of the most ............ gram-negative rod-shaped bacteria.
a) Multidrug resistant
b) Fastidious
c) Active
d) Infectious

14) Which of the following is a small, capnophilic, gram-negative, oxidase positive bacillus is frequently found in human bite infections.
a) Eikenella corrodens
b) Moraxella spp
c) Proteus vulgaris
d) Burkholderia mallie

15) Capnophiles are microorganisms that can grow in high concentrations of..............
a) Oxygen
b) Sugar
c) Carbon dioxide
d) pH

16) The isolation of this gram-negative bacteria associated with cystic fibrosis can be done by culturing the specimen in selective media containing colistin. A sputum sample is taken and cultured, colonies appear only after 72 hours of the incubation period, these bacteria are oxidase positive and further identification is done by using molecular methods, which bacteria is this?
a) Enterobacter aerogenes
b) Burkholderia cepacia
c) Pseudomonas aeruginosa
d) Acinetobacter johnsonii

17) …............is the part of the normal flora and is one of the most common causes of acute septic arthritis seen in children.
a) Moraxella catarrhalis
b) Staphylococcus saprophyticus
c) Cardiobacterium hominis
d) Kingella kingae

18) Which of the following gram-negative bacteria is a plant pathogen?
a) Pseudomonas syringae
b) Burkholderia glanders
c) Chryseobacterium spp
d) Capnocytophaga spp

19) Name the bacteria used as bioremediation agents which have the ability to degrade organic solvents such as toluene.
a) Pseudomonas syringae
b) Acinetobacter baumannii
c) Pseudomonas alcaligens
d) Pseudomonas putida

20) Pseudomonas aeruginosa is resistant to most of the antibiotics and this is mainly due to chromosomally encoded antibiotic resistance genes and the............ of the bacterial cellular envelopes.
a) Low susceptibility
b) High permeability
c) Low permeability
d) High susceptibility

Multiple Choice Answer Review
1-d) All species cause diseases in humans only
2-a) Green
3-b) Biofilm
4-a) 42 degree Celsius for 48 hours
5-d) All of the above
7-d)All of the above
8)- c) Phospholipase B
9-d) Acinetobacter baumannii
10-d) Causative agents of infectious endocarditis
11- d) Burkholderia pseudomallei
12-b) Pseudomonas aeruginosa
13-a) Multidrug resistant
14-a) Eikenella corrodens
15-c) Carbon dioxide
16-b) Burkholderia cepacia
17-d) Kingella kingae
18-a) Pseudomonas syringae
19-d) Pseudomonas putida
20-c) Low permeability




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