Microscopes: Simple and compound microscopes for microscopic examination, Microbiology, Biochemistry (Light microscopes/Optical microscopes, Inverted microscopes, Compound microscopes)
1. Simple microscopes Procedure : a) Set up the microscope Simple microscopes come with 4x, 10x, 25x and 40x objective lenses, use the desired magnifying lenses to observe and examine the microorganisms. Avoid touching the lenses on the objectives and the eyepieces. Plug in the microscope to turn the unit on. With both eyes open, look into the eyepieces. Adjust the interpupillary distance by holding the eyepiece tubes and rotate the eyepiece tubes either towards or away from each other until only one circle of light is seen by both eyes. Place the specimen to be studied onto the stage of the microscope (slide, flask, vial, etc). Using the mechanical stage’s slide controls, center the specimen over the stage opening, lining it up with the light and the objective lens. To adjust the illumination, turn the dimmer knob on the left side of the body until the desired intensity of light is achieved. Adjust the iris on the light source itself by r...