Lecture notes on CRISPR-Cas9 technology CRISPR-Cas9 technology is a revolutionary gene-editing tool that has had a profound impact on the field of genetics and molecular biology. The use of CRISPR-Cas9 for genome editing in microorganisms and its potential applications in medicine, agriculture, and biotechnology is an active area of research. In 2020, the Nobel Prize in Chemistry was conferred upon Emmanuelle Charpentier and Jennifer Doudna for their pioneering contributions to the development of CRISPR-Cas9 technology. For more information on CRISPR-Cas9: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9377665/ 1. What is CRISPR-Cas9? CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) and Cas9 (CRISPR-associated protein 9) are components of a bacterial immune system that helps bacteria defend against viral infections. Scientists have adapted this system to edi...