BIOCHEMISTRY, BIOMEDICINE & PHARMACEUTICS

Multiple Choice Questions on  Molecular Biology Techniques 1) DNA is the polydeoxynucleotides that contain mono-deoxynucleotides namely dAMP, dGMP, dCMP, dTMP.  Which one of the following is NOT true regarding DNA? a) DNA is double-stranded. b) The nucleotides are covalently linked by 3'-5' phosphodiester bond. c) Both strands of DNA are arranged parallel. d) DNA has a helical structure. 2) Phosphodiester bonds are cleaved by nucleases a group of enzymes present in the cells. Which of the following is FALSE about nucleases? a) Nucleases catalyze the hydrolytic cleavage of the phosphodiester linkages. b) Nucleases can be exonuclease and endonucleases. c) Exonucleases cleave the chain by removing individual nucleotides from the 3' or 5' end. d) Exonucleases cleave the chain randomly between any nucleotides. 3) Chromatin is a structural complex present in eukaryotes. The chromatin consists of the following components, EXCEPT: a) DNA b) RNA c) Histone pr...

       Acid capture elution (ACE) is a plate-based method for reducing the drug interference in the ADA (Adenosine deaminase) assay. It is used for the purification and isolation of specific nucleic acids, such as DNA or RNA, from a complex mixture. This method utilizes weak organic acids (Glycine, Acetic acid) to dissociate the drug-ADA complex.  The sample is neutralized and allowed the bind the plate coated with the drug. After the ADA is captured, the plate is washed to remove any unbound drug present in the sample.  The second acid treatment is used to elute the ADA bound to the plate.  The ADA sample is transferred, neutralized with Tris-HCl base, and processed in the ADA detection assay.  Procedure: 1) Binding (Coating of the plate with a drug): Maxisorp ELISA Nunc plate is widely used for coating the plate that would be used for the capture of ADA. For the optimization of drug concentration, test different concentrations of drug (0....