Skip to main content

MCQ on analytical methods used for for microbial identification and characterization: Microbiology, Biochemistry

                 MCQ on analytical methods used for for microbial identification

1. Which type of test involves analyzing the metabolic properties of microorganisms for identification?
a) Serological tests
b) Nucleic acid sequencing
c) Biochemical tests
d) MALDI-TOF mass spectrometry

2. Which of the following test uses antibodies to detect specific antigens on the surface of microorganisms for identification?
a) Enzyme-linked immunosorbent assays (ELISA)     
b) Phage typing
c) Polymerase chain reaction (PCR)
d) Whole Genome Sequencing (WGS)

3. Which analytical technique involves the amplification of specific DNA regions for detecting unique DNA sequences of microorganisms?
a) Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS)
b) Polymerase Chain Reaction (PCR)    
c) Phage typing
d) Serological tests

4. Which analytical technique targets a conserved region of the bacterial genome for microbial identification and classification?
a) 16S rRNA sequencing     
b) Gel electrophoresis
c) SDS-PAGE analysis
d) Western blotting

5. What is the main purpose of phage typing in microbial identification?
a) Identifying fatty acid composition
b) Detecting antigens
c) Analyzing protein profiles
d) Differentiating bacterial strains based on their susceptibility to viruses      

6. Which method rapidly identifies microorganisms by analyzing their unique protein profiles?
a) Phage typing
b) 16S rRNA sequencing
c) Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) 
d) DNA-DNA hybridization

7. Which of the following is correct statement regarding Whole Genome Sequencing (WGS)?
a) amplify specific DNA regions 
b) involves sequencing an organism's entire genome
c) involves using bacteriophages to differentiate bacterial strains based on their susceptibility to specific phages
d) uses mass spectrometry to analyze the unique protein profiles of microorganisms

8. FAME fatty acid methyl esters analysis involves converting the fatty acids from microbial cells into their methyl esters. The extracted FAMEs can be analyzed using which of the following of the following technique?
a) Gas chromatography (GC)
b) DNA-DNA hybridization
c) Enzyme-linked immunosorbent assays (ELISA)
d) Polymerase Chain Reaction (PCR)

9. Which automated system uses biochemical tests and other methods for microbial identification?
a) 16S rRNA sequencing
b) MALDI-TOF mass spectrometry
c) VITEK system
d) Western blotting

10. Which method is used to differentiate bacterial strains based on their susceptibility to specific bacteriophages?
a) 16S rRNA sequencing
b) Gas chromatography (GC)
c) MALDI-TOF mass spectrometry   
d) Phage typing 

Answers: 
1. c) Biochemical tests
2. a) Enzyme-linked immunosorbent assays (ELISA)
3. b) Polymerase Chain Reaction (PCR)    
4. a) 16S rRNA sequencing     
5. d) Differentiating bacterial strains based on their susceptibility to viruses  
6. c) Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS)    
7. b) involves sequencing an organism's entire genome
8. a) Gas chromatography (GC)
9. c) VITEK system
10. d) Phage typing




Comments

Popular posts from this blog

Carbohydrate Metabolism: MCQs and answers on Glycolysis & Gluconeogenesis

                                      MCQ on Glycolysis & Gluconeogenesis 1) Which of the following enzyme is not involved in galactose metabolism? a) Glucokinase b) Galactokinase c) Galactose-1-Phosphate Uridyl transferase d) UDP-Galactose 4- epimerase 2) Which of the following enzyme is defective in galactosemia (type I) - a fatal genetic disorder in infants? a) Glucokinase b) Galactokinase c) Galactose-1-Phosphate Uridyl transferase d) UDP-Galactose 4- epimerase 3) In the liver, the accumulation of which of the following metabolite attenuates the inhibitory of ATP on phosphofructokinase? a) Glucose-6-Phosphate b) Citrate c) Fructose-1,6-Bisphosphate d) Fructose-2,6-Bisphosphate 4) Mutation in which of the following enzymes leads to a glycogen storage disease known as "Tarui’s disease"? a) Glucokinase b) Phosphofructokinase c) Phosphoglucomutase d) Pyruvate Kinase 5) E...

MCQs and Answers on cultivation (culture/incubation), Isolation and Identification of microorganisms: Medical Microbiology

40 plus questions - Multiple Choice Questions on Classification, Culture, and Identification of the microorganisms 1. Which of the following microorganism has the cocci cell shapes and sizes arranged usually in tetrad structures? a)  Streptococcus pneumoniae b)  Staphylococcus aureus c)  Chlamydia trachomatis d)  Neisseria meningitidis 2. What are the different growth morphology and cell structures used for the classification of fungi? Select all the correct answers: a) Yeast b) Mold c) Mycelia d) Protozoa 3. Which of the following media is formulated with additional nutrients to support the growth of fastidious or nutritionally demanding bacteria that may not grow well on basic media? a) Differential media b) Enriched media c) Nutrient agar (media) d) Selective media 4. Which of the following metabolic characteristic is a distinguishing characteristic and identification of colonies of  E. coli ? a) Hydrogen sulfide formation b) Indole Formation c) Lactose fe...

Multiple Choice Questions (MCQs) on Diabetes Mellitus: Pathogenesis, Diagnosis and Treatment

                                        MCQs on Diabetes mellitus 1) Diabetes mellitus is a disorder characterized by hyperglycemia.  Which of the following is not the common characteristic features of type 2 diabetes mellitus ? a) Impaired insulin secretion b) Increased Insulin resistance  c) Diabetic ketoacidosis d) Excessive hepatic glucose production 2) Which of the following are the characteristic features of type 1 diabetes mellitus? a) Type 1 diabetes is caused by an absolute deficiency of insulin. b) Type 1 diabetes is associated with the autoimmune destruction of beta cells.  c) Patients with  uncontrolled type 1 diabetes present with diabetic ketoacidosis d) All of the above   3) Which of the following serum measurements are not used for the diagnosis of diabetes mellitus? a) Fasting blood glucose d) Postprandial blood glucose  c) Insulin ...