Skip to main content

Antibodies For Lysosomal LAMP1 Protein Western Blot Analysis & Immunofluorescence Staining

 LAMP1 lysosomal associated membrane protein 1 is widely used as Lysosome Marker

Lysosomal-associated membrane protein 1 is a glycoprotein from a family of Lysosome-associated membrane glycoproteins. The LAMP-1 glycoprotein is a type I transmembrane protein that resides primarily across lysosomal membranes, and functions to provide selectins with carbohydrate ligands. LAMP proteins are responsible in part for maintaining lysosomal integrity, pH, and catabolism. The expected molecular weight of the protein is approximately 110kDa.

Antibodies for detecting LAMP 1 Proteins using Western Blot & Immunofluorescence Analysis (Peer-Reviewed Publications)

LAMP1 is routinely used as a lysosome marker and LAMP1-positive organelles are often referred to as lysosomal compartments. Below are the antibodies against LAMP 1 used in the peered reviewed publications.
LAMP1 Staining (Rostami et al. 2020)
Mouse anti-human LAMP-1 (D401S; Cell Signaling Technology): Monoclonal antibody is produced by immunizing animals with a recombinant protein fragment of human LAMP1 protein. LAMP1 (D4O1S) Mouse mAb recognizes endogenous levels of total LAMP1 protein.
Dilution 1:100 (Details)

Alexa Fluor® 488 anti-mouse CD107a (LAMP-1) Antibody (Biolegend, San Diego, CA): This monoclonal antibody was raised against NIH/3T3 mouse embryonic fibroblast tissue culture cell membranes. It has been mapped to the N-terminus of LAMP-1. 
Dilution & Applications (Details)

Anti-LAMP1 (1D4B, DSHB, Iowa City, IA, USA): This monoclonal antibody was raised against NIH/3T3 mouse embryonic fibroblast tissue culture cell membranes. 
Dilution & Applications (Details)

Anti-LAMP1 (D2D11, XP® Rabbit mAb #9091 Cell Signalling Technology, Danvers, MA, USA): LAMP1 (D2D11) XP® Rabbit mAb recognizes endogenous levels of total LAMP1 protein. A monoclonal antibody is produced by immunizing animals with a recombinant protein fragment of human LAMP1 protein. 
Dilution & Applications  (Details)

Easy to Follow Protocol for Western Blot for Detection of LAMP1 Protein

References

Comments

Popular posts from this blog

Carbohydrate Metabolism: MCQs and answers on Glycolysis & Gluconeogenesis

                                      MCQ on Glycolysis & Gluconeogenesis 1) Which of the following enzyme is not involved in galactose metabolism? a) Glucokinase b) Galactokinase c) Galactose-1-Phosphate Uridyl transferase d) UDP-Galactose 4- epimerase 2) Which of the following enzyme is defective in galactosemia (type I) - a fatal genetic disorder in infants? a) Glucokinase b) Galactokinase c) Galactose-1-Phosphate Uridyl transferase d) UDP-Galactose 4- epimerase 3) In the liver, the accumulation of which of the following metabolite attenuates the inhibitory of ATP on phosphofructokinase? a) Glucose-6-Phosphate b) Citrate c) Fructose-1,6-Bisphosphate d) Fructose-2,6-Bisphosphate 4) Mutation in which of the following enzymes leads to a glycogen storage disease known as "Tarui’s disease"? a) Glucokinase b) Phosphofructokinase c) Phosphoglucomutase d) Pyruvate Kinase 5) E...

MCQs and Answers on cultivation (culture/incubation), Isolation and Identification of microorganisms: Medical Microbiology

40 plus questions - Multiple Choice Questions on Classification, Culture, and Identification of the microorganisms 1. Which of the following microorganism has the cocci cell shapes and sizes arranged usually in tetrad structures? a)  Streptococcus pneumoniae b)  Staphylococcus aureus c)  Chlamydia trachomatis d)  Neisseria meningitidis 2. What are the different growth morphology and cell structures used for the classification of fungi? Select all the correct answers: a) Yeast b) Mold c) Mycelia d) Protozoa 3. Which of the following media is formulated with additional nutrients to support the growth of fastidious or nutritionally demanding bacteria that may not grow well on basic media? a) Differential media b) Enriched media c) Nutrient agar (media) d) Selective media 4. Which of the following metabolic characteristic is a distinguishing characteristic and identification of colonies of  E. coli ? a) Hydrogen sulfide formation b) Indole Formation c) Lactose fe...

Multiple Choice Questions (MCQs) on Diabetes Mellitus: Pathogenesis, Diagnosis and Treatment

                                        MCQs on Diabetes mellitus 1) Diabetes mellitus is a disorder characterized by hyperglycemia.  Which of the following is not the common characteristic features of type 2 diabetes mellitus ? a) Impaired insulin secretion b) Increased Insulin resistance  c) Diabetic ketoacidosis d) Excessive hepatic glucose production 2) Which of the following are the characteristic features of type 1 diabetes mellitus? a) Type 1 diabetes is caused by an absolute deficiency of insulin. b) Type 1 diabetes is associated with the autoimmune destruction of beta cells.  c) Patients with  uncontrolled type 1 diabetes present with diabetic ketoacidosis d) All of the above   3) Which of the following serum measurements are not used for the diagnosis of diabetes mellitus? a) Fasting blood glucose d) Postprandial blood glucose  c) Insulin ...