LAMP1 lysosomal associated membrane protein 1 is widely used as Lysosome Marker
Lysosomal-associated membrane protein 1 is a glycoprotein from a family of Lysosome-associated membrane glycoproteins. The LAMP-1 glycoprotein is a type I transmembrane protein that resides primarily across lysosomal membranes, and functions to provide selectins with carbohydrate ligands. LAMP proteins are responsible in part for maintaining lysosomal integrity, pH, and catabolism. The expected molecular weight of the protein is approximately 110kDa.
Antibodies for detecting LAMP 1 Proteins using Western Blot & Immunofluorescence Analysis (Peer-Reviewed Publications)
LAMP1 is routinely used as a lysosome marker and LAMP1-positive organelles are often referred to as lysosomal compartments. Below are the antibodies against LAMP 1 used in the peered reviewed publications.
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| LAMP1 Staining (Rostami et al. 2020) |
Mouse anti-human LAMP-1 (D401S; Cell Signaling Technology): Monoclonal antibody is produced by immunizing animals with a recombinant protein fragment of human LAMP1 protein. LAMP1 (D4O1S) Mouse mAb recognizes endogenous levels of total LAMP1 protein.
Dilution 1:100 (Details)
Alexa Fluor® 488 anti-mouse CD107a (LAMP-1) Antibody (Biolegend, San Diego, CA): This monoclonal antibody was raised against NIH/3T3 mouse embryonic fibroblast tissue culture cell membranes. It has been mapped to the N-terminus of LAMP-1.
Dilution & Applications (Details)
Anti-LAMP1 (1D4B, DSHB, Iowa City, IA, USA): This monoclonal antibody was raised against NIH/3T3 mouse embryonic fibroblast tissue culture cell membranes.
Dilution & Applications (Details)
Anti-LAMP1 (D2D11, XP® Rabbit mAb #9091 Cell Signalling Technology, Danvers, MA, USA): LAMP1 (D2D11) XP® Rabbit mAb recognizes endogenous levels of total LAMP1 protein. A monoclonal antibody is produced by immunizing animals with a recombinant protein fragment of human LAMP1 protein.
Dilution & Applications (Details)
Easy to Follow Protocol for Western Blot for Detection of LAMP1 Protein
References