Skip to main content

Agilent Agilent Rapid Fire HTS Technology for Determination of Serum Biomarkers (Amino Acids) For Gene Therapy

Agilent RapidFire 400

The Agilent RapidFire 400 is an integrated autosampler for ultrafast sample cleanup based on automated solid-phase extraction (SPE) and introduction to mass spectrometry. Designed for highest-throughput laboratories, the large capacity, temperature-controlled, 1536 well-plate handling robot allows unattended weekend operation with sample injection times as short as 8 seconds. RapidFire 400 builds on successful previous models, combining more than 14 years of high-throughput mass spectrometry innovation.

This system provides reliable high throughput and accurate measurements for the qualification of drug targets like automated ADME in discovery, compound binding assessment, elucidation of metabolic processes, the discovery of novel metabolomics targets, and much more. Fully controlled by a single PC, the RapidFire 400 can be combined with any Agilent LC/Q-TOF or LC/TQ, while integrating the Ultivo LC/TQ into the system dramatically reduces the footprint of the autosampler and mass spec combination.


Application: 
  • Studying ADME for drug discovery
  • Measurement of Phenylalanine and Tyrosine for PKU Gene Therapy (Homology Therapeutics)
  • Study posttranslational modification of proteins 
Reference: 
Ahmed et al, 2020, Methods and Clinical Development
Agilent 

Comments

Popular posts from this blog

Carbohydrate Metabolism: MCQs and answers on Glycolysis & Gluconeogenesis

                                      MCQ on Glycolysis & Gluconeogenesis 1) Which of the following enzyme is not involved in galactose metabolism? a) Glucokinase b) Galactokinase c) Galactose-1-Phosphate Uridyl transferase d) UDP-Galactose 4- epimerase 2) Which of the following enzyme is defective in galactosemia (type I) - a fatal genetic disorder in infants? a) Glucokinase b) Galactokinase c) Galactose-1-Phosphate Uridyl transferase d) UDP-Galactose 4- epimerase 3) In the liver, the accumulation of which of the following metabolite attenuates the inhibitory of ATP on phosphofructokinase? a) Glucose-6-Phosphate b) Citrate c) Fructose-1,6-Bisphosphate d) Fructose-2,6-Bisphosphate 4) Mutation in which of the following enzymes leads to a glycogen storage disease known as "Tarui’s disease"? a) Glucokinase b) Phosphofructokinase c) Phosphoglucomutase d) Pyruvate Kinase 5) E...

MCQs and Answers on cultivation (culture/incubation), Isolation and Identification of microorganisms: Medical Microbiology

40 plus questions - Multiple Choice Questions on Classification, Culture, and Identification of the microorganisms 1. Which of the following microorganism has the cocci cell shapes and sizes arranged usually in tetrad structures? a)  Streptococcus pneumoniae b)  Staphylococcus aureus c)  Chlamydia trachomatis d)  Neisseria meningitidis 2. What are the different growth morphology and cell structures used for the classification of fungi? Select all the correct answers: a) Yeast b) Mold c) Mycelia d) Protozoa 3. Which of the following media is formulated with additional nutrients to support the growth of fastidious or nutritionally demanding bacteria that may not grow well on basic media? a) Differential media b) Enriched media c) Nutrient agar (media) d) Selective media 4. Which of the following metabolic characteristic is a distinguishing characteristic and identification of colonies of  E. coli ? a) Hydrogen sulfide formation b) Indole Formation c) Lactose fe...

MCQs on Bacterial Pathogenesis: Medical Microbiology

Key aspects of Bacterial pathogenesis/ General steps in bacterial pathogenesis Adherence and Colonization  Invasion Immune Evasion Toxin Production Nutrient Acquisition Host Cell Manipulation Inflammation and Tissue Damage                         Multiple Choice Questions on Bacterial Pathogenesis       Figure 1: Phagocyte activity and possible outcomes of phagocyte-bacterial interactions                                     (Source/Reference: Bailey & Scott’s Diagnostic  Microbiology) 1) Which of the following microorganism is the major inhabitant of the human skin? a) Escherichia coli b) Staphylococcus epidermidis c) Staphylococcus aureus d) Streptococcus pyogenes 2) Select all the medically important bacteria which are frequently isolated pathogen  human infections? a) Enteric ba...