Surface Plasmon ResonanceSurface Plasmon Resonance (SPR) is an optical technique used to measure molecular interactions in real-time. SPR can occur when plane-polarized light hits a metal film under total internal reflection conditions. SPR signal is directly dependent on the refractive index of the medium on the sensor chip. The binding of biomolecules results in changes in the refractive index on the sensor surface.
In an SPR experiment, one molecule (the Ligand) is immobilized on a sensor chip and binding to a second molecule (the Analyte) is measured under flow. Response is measured in resonance units (RU) and is proportional to the mass on the surface, and for any given interactant, the response is proportional to the number of molecules bound to the surface. Response is recorded and displayed as a sensogram in real time.
Principle of Surface Plasmon Resonance (GE)
Application of SPR
- Increase understanding of molecular mechanisms and structure-function relationships
- Define potential drug targets and diagnostic markers
- Select and characterize biotherapeutic candidates
- Select and optimize lead compounds during drug discovery
- Detect and characterize anti-drug antibodies (ADA) in immunogenicity studies
- Comprehensive characterization of ADAs, detection of IgG4, determination of epitope specificity
- Perform time- and cost-efficient concentration analysis in vaccine development
Popular SPR Platforms Choices For Your Laboratory
Biacore™ T200 (GE)
Biacore T200 (Fig 1) is a versatile, label-free system for detailed studies of biomolecular interactions, from early research to drug discovery and development, and on to QC. The system delivers high quality kinetic, affinity, concentration, specificity, selectivity, and thermodynamic interaction data – in real-time with exceptional sensitivity. Interactions characterized by on- and off-rates at the extremities of the kinetic scale can be analyzed with greater precision and confidence than ever before. Kinetics • Biacore allows the characterization of affinity, specificity, concentration & Relative binding
Carterra® LSATM Instrument
The LSA is the only fully integrated, high throughput mAb screening and characterization platform that combines patented flow printing microfluidics with high throughput surface plasmon resonance (SPR) detection to deliver high throughput kinetics and epitope discovery applications to support state-of-the-art mAb discovery programs. Unique fluidics integrates both single flow cell and 96-channel printhead modes with high throughput SPR.
OpenPlex is the ideal solution for the development of label-free multiplexed bioassays and biomolecules detection. It uses Surface Plasmon Resonance imaging to track the real-time binding of molecules on multiple spotted areas located on a sensor chip. Typical applications include biosensor development, pathogen detection, water monitoring, nanotechnology, and surface characterization
Openplex allows the characterization of affinity, specificity, concentration & Relative binding.
Reichert has a four-channel surface plasmon resonance (SPR) system that provides results equivalent to the leading competitor, with a 30-70% lower total cost of ownership over five years. The Reichert4SPR System pushes the limits of detection and sensitivity for label-free biomolecular interaction analysis, and have been used in leading universities, academic facilities and biotech startups around the world.
Sierra SPR-24 Pro
Sierra SPR-24 Pro is a high performance, high throughput SPR analytical biosensor for the real-time, label-Free Characterization of Molecular Interactions. SPR-24 Pro allows screening, kinetics study, epitope binning, and thermodynamics. Capabilities include 4400+ samples assayed per day, full kinetic analysis of 2 interactions per assay cycle, 8 multi-control analyses per cycle industry.