Laboratory diagnosis of the diseases caused by bacteria, fungi, and viruses- culture methods and identification

 MCQ on Laboratory diagnosis of the diseases caused by bacteria, fungi, and viruses

1) Select all the important procedures that are required when performing the routine collection method of microbial specimens
a. A specimen should be collected in a sterile container
b. A specimen should be properly labeled with the patient name, date, and time of collection
c. A specimen should be collected by using a sterile cotton swab or collection needles 
d. A specimen should be collected after the start of antibiotic therapy 

2) Which of the following is the correct volume size of blood specimen taken for the routine laboratory diagnosis of the infection and the identification of the possible pathogen?
a. 1 ml
b. 10 ml     
c. 20 ml
d. 0.5 ml

3) Which of the following specimen is appropriate and commonly used for the proper diagnosis of Salmonellosis? Select all the correct options
a. Blood specimen     
b. Throat swab
c. Urine specimen
d. Stool specimen     

4) Which of the following specimen collection procedure can give accurate results for the laboratory diagnosis of Tuberculosis?
a. Throat swab specimen each morning for 3 consecutive days
b. Sputum specimen each morning for 3 consecutive days      
c. Blood specimens once a week for 2 weeks
d. Skin biopsy test before the start of antibiotics therapy

5) Name the routine method used for the direct microscopic examination of a urine sample taken from a person suspected of urinary tract infection?
a. Wet mount method
b. Hanging drop method
c. Potassium hydroxide method
d.  

6) Herpes simplex virus is one of the contagious viral diseases, HSV 1 is can spread from person to person. 
Which of the following cells appear as multinucleated cells taken from the lesions specimen and can be observed by the direct microscopy method?
a. 
b. Buruli ulcer cells
c. Schizont cells
d. Tzanck cells     

7) Which of the following temperature can be optimal for the storage of swab specimens taken from the Coronavirus infected patient while performing the Covid-19 test method? 
a. 2 to 4 degrees Celsius for up to 24 hours after the specimen collection
b. 25 degrees celsius for up to 3 days after the specimen collection
c. 2 to 4 degrees Celsius for up to 72 hours after the specimen collection     
d. 5 to 10 degrees Celsius for up to a week after the specimen collection

8) Which of the following microorganism can be detected by their ability to produce a toxin and can be identified by Elek's test method?
a. Cryptococcus neoformans
b. Corynebacterium diphtheria     
c. Mycobacterium tuberculosis
d. Bacillus anthracis

9) Which of the following chemical compound is commonly used to clean and decontaminate the infected skin area or nail before the collection of the specimens and for the laboratory diagnosis?
a. Iodine solution
b. KOH solution
c. Warm water
d. 70% alcohol     

10) Which of the following is the most widely used test for the detection of genetic material in the Covid-19 swab sample?
a. PCR test (Polymerase chain reaction)      
b. Western blot test
c. ELISA (Enzyme-linked immunosorbent assay)
d. Animal inoculation test

11)


Answers:
1) a., b., and c
2) 10 ml
3) a.Blood specimen and d. Stool specimen
4) Sputum specimen each morning for 3 consecutive days
5) Wet mount method
6) d. Tzanck cells
7) c. 2 to 4 degrees Celsius for up to 72 hours after the specimen collection 
8) b. Corynebacterium diptheriae
9) d. 70% alcohol
10) a. PCR test























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