Analytical Platform Review: ELISpot for Study T cell Response

The enzyme-linked immunospot (ELISpot) assay has been widely used for studying cellular immunology, providing both quantitative and qualitative information on cellular cytokine responses to defined antigens. It enables the comprehensive screening of patient-derived peripheral blood mononuclear cells to detect the antigenic restriction of T-cell responses for infectious diseases. This technology is has emerged as the gold standard for identifying T cell-mediated response against viral capsid protein and transgenes. 

Principle of ELIspot



ELISpot Platform Suitable For Your Laboratory

 Immunspot CTL S6 Analyzer 

ImmunoSpot® Analyzers combine accuracy, flexibility, and scientific methodology, making them invaluable research tools. In addition to ELISPOT assays, the analyzer can also be used for analyzing other microtiter plate-based bioassays. These include bacterial, yeast, stem cell, and tumor colonies, as well as viral plaque assays and more.
In addition to providing high-resolution visible light analysis, multiple fluorescent light sources enable fluorescent applications — FluoroSpot, cell cycle analysis, cell viability tests, apoptosis tests etc. The automated filter changer allows up to 8 wavelengths of fluorescent analysis.

AID vSpot Spectrum (VSR078IFL)

The AID vSpot Spectrum is the new high-end EliSpot/FluoroSpot device from AID. It combines AID iSpot 96-well FluoroSpot analyzing with enzymatic multiple plate evaluation. On the enzymatic side the AID vSpot Spectrum can handle a variety of different assay types including Viral Plaque Assays and Neutralization Assays. It will also read 6, 12, 24, 48, and 384-well plates. The insertion of high-resolution digital cameras provides well images of unprecedented quality. The capabilities of this technology include EliSpot, FluoroSpot, Virus Plaque Assays, Colony Counting etc.

Mabtech IRIS - Spot analysis

A next-generation, 4-color FluoroSpot, and ELISpot reader using RAWspotTM technology that ensures reliable multiplexing. A new dimension of data The proprietary RAWspotTM technology extracts the volume of every spot, which corresponds to the relative amount of secreted analyte.FluoroSpot combines the sensitivity of ELISpot with the capacity to analyze the secretion of several analytes simultaneously. This highly sensitive cellular assay is robust, easy to perform, and suitable for both single tests and large-scale screening. The capabilities include:
  • Capture- analyte secretions at the single-cell level. 
  • Detect immune responses without manipulation of intracellular processes.
  • Discover the true potential of FluoroSpot and see a new dimension in your research. 

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