Analytical Platform Review: Mass Spectrometers (LC-MS) for Protein Characterization and Quantification

Liquid Chromatography-Mass Sppectoromtery-

It is a powerful analytical technique that combines the resolving power of liquid chromatography with the detection specificity of mass spectrometry. Liquid chromatography (LC) separates the sample components and then introduces them to the mass spectrometer (MS). The MS creates and detects charged ions. The LC/MS data may be used to provide information about the molecular weight, structure, identity, and quantity of specific sample components.

Applications of LC-MS

  • Proteomics
  • Metabolomics
  • Protein Identification, Characterization and Quntitation
  • Protein Structural Analysis

Triple quadrupole Mass Spectrometry Principle (Watch Video)

6470B Triple quadrupole LC/MS (Agilent)

Technical Specification
The Agilent 6470B triple quadrupole LC/MS system delivers robust performance and high versatility for commercial, life sciences research, and regulatory applications. It has best-in-class sensitivity, precision, and scan speed. 
The 6470B LC/MS/MS triple quadrupole employs multiple technological advantages like the Agilent Jet Stream ion source, curved geometry collision cell, and ±20 kV high energy dynode. These innovations maximize ionization, minimize instrument downtime, and reach extremely low limits of detection while with high accuracy and broad dynamic range. 

Sciex QTRAP 6500 (Sciex)

QTRAP is the only triple quadrupole technology in the world powered by a linear ion trap (LIT), combining the MRM sensitivity of a triple quadrupole system but enhanced with unique and powerful workflows for better data, complete confidence, unrivaled efficiency, and ultimate performance.
The features of QTRAP include 
Multiple Reaction Monitoring (MRM)
Enhanced Product Ion (EPI)
Enhanced MS (EMS)
Enhanced Multiply Charged (EMC)
Enhanced Resolution (ER)

Thermo Scientific TSQ Altis

Thermo Scientific TSQ Altis is a triple-stage quadrupole mass spectrometer that has high sensitivity, selectivity, and rapid turnaround with reproducibility and robustness.It features segmented quadrupoles with hyperbolic faces, an enhanced dual-mode discrete-dynode electron multiplier detector, and high-resolution SRM (0.2 FWHM). 

Shimadzu LCMS-8060

By redefining the ion focusing capability on the UF-Qarray™, the LCMS-8060 brings a meaningful impact on quantitative detection and opens new opportunities for scientists to deliver solutions that make a true difference. The newly developed UF-Qarray ion guide is a groundbreaking technology that increases LC/MS/MS sensitivity by enhancing ion signal intensity and reducing noise. By improving the ion sampling device, the ion guide, and the vacuum efficiency, the LCMS-8060 delivers a new vision of sensitivity and makes a real difference in working better and faster

Time of Flight(TOF) Mass Spectrometry Principle (Watch Video)

6545XT AdvanceBio LC/Q-TOF (Agilent)

Technical Specification
The Agilent 6545XT AdvanceBio LC/Q-TOF system is designed to handle multiple workflows in biopharmaceutical characterization. The instrument is most suitable to study and quantify the intact protein level, peptide mapping, and PTMs using UHPLC-Q-TOF. 
Accurately profile major and minor proteoforms with time-of-flight technology, offering new levels of sensitivity and clarity to intact protein mass spectrometry. The 6545XT AdvanceBio Q-TOF is the analytical cornerstone in a comprehensive set of tools designed for biopharma.

LCMS-9030 Q-TOF Mass Spectrometer (Shimadzu)

Technical Specification
The LCMS-9030 uses newly patented technologies to deliver both high resolution and accurate mass, attributes essential for confident formula assignment, and unknown identification. High-efficiency ion guides, quadrupole, and collision cell enable high sensitivity for the detection of trace-level compounds. Unique UFgrating and iRefTOF technologies ensure ultrafast acceleration of ions into the flight tube (UF-FlightTube) and ideal reflection of those ions back to the detector. The result is high-speed data acquisition compatible with the high-throughput laboratory.

Sciex TripleTOF® 6600 System

TripleTOF 6600+ System offers sensitive and robust quantitation using dedicated low flow source technology. Redesigned TOF entrance optics, along with software improvements within the forthcoming Analyst® TF Software 1.8, deliver optimized ion beam control, detector tuning, and scheduled ionization to decrease the risk of downtime over long term usage.


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