BIOCHEMISTRY, BIOMEDICINE & PHARMACEUTICS

Linearity: The alternative approach for detecting non-linearity is to assess the residuals of an estimated regression line and test for whether positive and negative deviations are randomly distributed. It should be considered whether an estimated regression line passes through zero or not. The presence of linearity is a prerequisite for a high degree of trueness. Linearity refers to the relationship between measured and expected values over the analytical measurement range. Linearity may be considered in relation to the actual or relative analyte concentration. In case of relative analyte concentrations, a dilution series of a sample may be studied. The relative analyte concentration method is often used for immunoassays to measure whether the concentration decreases with dilution as expected. Dilution is usually carried out with the appropriate sample matrix. Analytical Range: The analytical range or reportable range is the analyte concentration range over which the measurement i...

The surfactants are chemical substance that is capable of lowering surface tension. The lung surfactants contain a complex mixture of phospholipids, neutral lipids, and specific proteins. The lung surfactants are essential for normal lung function as it reduces surface tension at the air-liquid interface of alveolar spaces.  Phosphatidylcholine is a major component of the surfactant comprising 90% of the lipids. Four specific surfactant proteins SP-A, SP-B, SP-C, SP-D. The synthesis and secretion of functional surfactants are confined to type II cells of the alveolar epithelium. The fundamental pathway for lung surfactant phospholipid synthesis, it's packaging into storage lamellar bodies (LB), secretion into the alveolar lining fluid and recycling back into ATII cells are well known. Bulk surfactant phospholipid synthesized at the ER is transported to LBs by a mechanism that may employ specific carrier proteins and/or traffic through the Golgi.  During the processes of s...